MAP2抗体
Applications
| In-house applications |
ELISA, WB, IF, IP, IHC Application not tested? Please see our Guarantee. |
| Published applications |
FC, IF, IHC, WB |
Publications |
14 citations –view here |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
| Published Species |
Human, mouse, rat |
|
Images
Prev12Next
 Immunohistochemical of paraffin-embedded human brain using 17490-1-AP(MAP2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. Immunohistochemical of paraffin-embedded human brain using 17490-1-AP(MAP2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17490-1-AP(MAP2 antibody) at dilution of 1:1000 IP Result of anti-MAP2 (IP:17490-1-AP, 3ug; Detection:17490-1-AP 1:1000) with mouse brain tissue lysate 5000ug. Immunofluorescent analysis of Hela cells, using 17490-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).
|
| Positive WB detected in |
SH-SY5Y cells, mouse brain tissue |
| Positive IP detected in |
Mouse brain tissue |
| Positive IHC detected in |
Human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IF detected in |
Hela cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:20-1:400
IF : 1:10-1:100 |
MAP2抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen |
MAP2 fusion protein ag11580 |
Full name |
Microtubule-associated protein 2 |
Calculated molecular
weight |
559aa,59 kDa |
Observed molecular
weight |
280 kDa, 70-85 kDa |
GenBank accession
number |
BC038857 |
Gene ID (NCBI) |
4133 |
| Gene symbol |
MAP2 |
Synonyms |
DKFZp686I2148, MAP 2, MAP2, MAP2A, MAP2B, MAP2C |
Background MAP2抗体
|
MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in brain and has important role in neuronal morphogenesis. Multiple high molecular weight (MW) and low molecular weight (MW) MAP2 isoforms are expressed within axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally specific manner. The 280 kD MAP2B is present throughout rat brain development, and the slightly larger MAP2A appears first during the end of the second week of postnatal life. MAP2C, composed of several bands of about 70 kD, is present during early brain development, and largely disappears from the mature brain except for the retina, olfactory bulb, and cerebellum. MAP2 antibodies have been widely used to mark the neuron or dendrite formation. This antibody can recognize both high MW and low MW isoforms of MAP2. MAP2抗体
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