Histone H3 (K4me2) polyclonal antibody
产品名称： Histone H3 (K4me2) polyclonal antibody
英文名称： Histone H3 (K4me2) polyclonal antibody
产品编号： PAB15453
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K4me2).
- Immunogen:
- Lysine4 dimethylated peptide of Histone H3.
- Host:
- Rabbit
- Reactivity:
- Yeast
- Specificity:
- This antibody is expected to react with Histone H3 from yeast to mammals.
- Form:
- Liquid
- Storage Buffer:
- In PBS, pH 7.2 (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
- ChIP
- Histone H3 (K4me2) polyclonal antibody (Cat # PAB15453) was used in ChIP with murine embryonic stem cells (Lane 1) or erythroleukemia cells (Lane 2).
Top Panel : PCR primers specifc for beta-globulin were used to amplify a 210 base pair promoter following DNA isolation.
Lower Panel : Input DNA control.
- Western Blot (Cell lysate)
- Acid treated HeLa (10 ug) extract was treated with a 1 : 500 dilution of antibody Histone H3 (K4me2) polyclonal antibody (Cat # PAB15453) prior to visualization.
- Dot Blot (Peptide)
- To confrm the specifcity of the antisera a dot blot system was used with amounts of peptides from 10-250 pmoles.
Unmodifed, monomethylated, dimethylated and trimethylated peptides surrounding the Lysine 4 site on Histone H3 were spotted in lanes 1-4 respectively.
Lanes 5-8 contained peptides starting at residue 6, with the sequence, TARKSTGGKAPRKQLAT, encompassing the residue lysine 9 on Histone H3 that is unmodifed, monomethylated, dimethylated and trimethylated respectively.
Lanes 9-12 contained peptides encompassing the residue lysine 27 on Histone H3 that is unmodifed, monomethylated, dimethylated and trimethylated respectively.